Table 1.

Composition of the Earle’s balanced salt solution (EBSS) medium^*

Component	Concentration (mmol/L)
Anhydrous CaCl2	1.050
CuSO4∙5H2O	5.2×10−6
Fe(NO3)3∙9H2O	1.238×10−4
FeSO4∙7H2O	0.002
Anhydrous MgCl2	0.301
Anhydrous MgSO4	0.407
KCl	4.157
NaHCO3	14.286
NaCl	120.612
Anhydrous Na2HPO4	0.500
NaH2PO4∙H2O	0.453
ZnSO4∙7H2O	0.002
D-Glucose (dextrose)	17.506
Phenol red	0.022

^*Modified from Gao et al. (2015)