Table 2.

Leu levels in the medium for the in vitro culture of CMEC-H from dairy cattle

Group	EBSS	Leu (mmol/L)	10% FBS
Negative control	+
Positive control	+		+
Treatment	+	0.45
	+	1.35
	+	5.40
	+	10.80

DMEM/F12 base media contained leucine (0.45 mmol/L) at a level sufficient for normal cellular processes. +: add