Table 3.

His levels in the medium for the in vitro culture of CMEC-H from dairy cattle

Group	EBSS	His (mmol/L)	10% FBS
Negative control	+
Positive control	+		+
Treatment	+	0.15
	+	1.20
	+	4.80
	+	9.60

DMEM/F12 base media contained histidine (0.14999 mmol/L) at a level sufficient for normal cellular processes. +: add