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. 2015 Jun 18;5:11275. doi: 10.1038/srep11275

Figure 2. Effect of vaccines on splenic T lymphocytes in tumour-bearing mice.

Figure 2

Cell suspensions were derived from spleens of mice immunized over four cycles with PBS (A), Adv (B), Adv-m (C), AdVEGFR2-m (D), AdTERT-m (E) or Ad(VEGFR2:TERT)-m (F). After reaction with mAb to CD4 and CD8, the suspension was subjected to flow cytometry. The proportion of CD8+ and CD4+ T cells in the vaccinated groups (B–E) was markedly higher than that in control (A). Immunotherapy with the AdVEGFR2-m vaccine alone (D) increased the number of CD4+ T cells (p = 0.006), while the AdTERT-m vaccine alone (E) increased the number of CD8+ T cells (p = 0.048) compared to the PBS, Adv and Adv-m groups (A-C) respectively. Furthermore, immunotherapy with the combination increased both the number of CD4+ T cells (p = 0.009) and CD8+ T cells (p = 0.034) in the spleen (F); (G) presents a bar graph.