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. 2015 Jun 18;5:11275. doi: 10.1038/srep11275

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Copyright © 2015, Macmillan Publishers Limited

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Frozen sections of B16 tumour tissues isolated from mice immunized with PBS (A), Adv (B), Adv-m (C), AdVEGFR2-m (D), AdTERT-m (E) and Ad(VEGFR2:TERT)-m (F) were stained with an antibody reactive to CD31. The vessel density of each sample was estimated by averaging the total number of microvessels in five random fields (brown; original magnification, ×200). The vessel density in the Ad (VEGFR2:TERT)-m group was significantly lower than that in the other groups (G) (p < 0.05, variance test).