Figure 3.

PJE impairs HCC cell growth through the inhibition of the Akt/mTOR and Wnt signaling pathway in vitro and in vivo. (A) With increasing concentrations of PJE, the expression levels of p-mTOR, p-Akt, β-catenin and p-GSK3β were shown to significantly decrease in the Hep3B cells. (B) PJE group exhibited reduced tumor growth compared with the control group. (C) Western blot analysis of each tumor lysate at the end of the experiment. PJE, methanol extract of Petasites japonicus; HCC, hepatocellular carcinoma; p, phosphorylated; GSK, glycogen synthase kinase.