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Isolation and identification of umbilical cord mesenchymal stem cells (UCMSCs). (A) Appearance of UCMSCs after passaging (scale bar, 100 µm). (B) Identification of third-passage UCMSCs revealed positive expression of CD44 (97.1%) and CD90 (95.8%), and negative expression of CD34 (3.0%) and CD45 (0.04%), as analyzed by flow cytometry. FITC, fluorescein isothiocyanate.
