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. 2015 Apr 22;106(6):709–717. doi: 10.1111/cas.12662

Figure 7.

Figure 7

Knockdown of CRK inhibits tumor growth and circulating tumor cells, and completely abolishes BC metastasis in vivo. UM-UC-3 cells labeled with tdTomato-luc2 (control, empty; CRK depletion, CRKi-3) were orthotopically injected under the bladder muscle layer in athymic mice (n = 5 each group). (a,b) Tumor growth was measured weekly by a bioluminescence imaging system, and graphed as mean ± SE from five mice in each group (b). (c) After 48 days, the mice were killed and the weight of the excised primary bladder tumors was measured. (d) After 48 days, bioluminescent imaging of photons from blood (100 μL), and liver and lung metastatic tumor burden was taken ex vivo (left) and plotted (right). (e) Excised tumors were fixed and stained with H&E. In mice injected with CRKi cells, the metastasis to the liver and lung was absent. (f) Primary bladder tumors were subjected to immunostaining for CRK, MIB-1, and MMP9. *P < 0.05, **P < 0.01.