Table 3.

CD and SEC-MALS experimental details

DOI: http://dx.doi.org/10.7554/eLife.07410.028

CD
Protein name	Concentration (μM)
SAS-5FL	1.5
SAS-5Δ282–295	3
SAS-5FLEX	3
SAS-52–265	6
SAS-52–265 L141E	6
SAS-52–265 L141E/M167E	6
SAS-5–265 I247E	6
SAS-52–265 L141E/I247E	6
SAS-5125–265	10
SAS-5CC	20
SAS-5CC L141E	20
SAS-5Imp	20
SAS-5Imp I247E	20
SAS-5 N-terminus	20
SAS-5 C-terminus	5

SEC-MALS
Protein name	Monomeric MW (kDa)	Size exclusion column	Minimum concentration (μM)*	Maximum concentration (μM)*
SAS-5FL	46	Superose6	0.10	0.57
SAS-5FLEX-MsyB	62	Superose6	0.03	9.5
SAS-52–265-MsyB (Figure 4)	46	Superose6	0.02	41
SAS-52–265-MsyB (Figure 5)	46	Superdex200	0.05	41
SAS-52–265 L141E	46	Superdex200	1.32	63
SAS-52–265 L141E/M167E	46	Superdex200	1.16	63
SAS-52–265 I247E	46	Superdex200	0.05	63
SAS-52–265 L141E/I247E	46	Superdex200	0.82	63
SAS-5Imp	6.5	Superdex75	2.9	292
SAS-5CC-L	12.5	Superdex75	0.18	152
C. briggsae SAS-5 (96–199)	12.7	Superdex75	0.10	149
C. brenneri SAS-5 (1–82)	10.2	Superdex75	0.21	186
C. remanei SAS-5 (98–206)	13	Superdex75	0.21	146
C. sinica SAS-5 (94–204)	13	Superdex75	0.16	145
C. tropicalis SAS-5 (94–199)	12.5	Superdex75	0.12	152

^*Refers to on-column concentration calculated from the protein differential refractive index.