Figure 6.

Sodium Nitroprusside Promotes Generation of Cardiac Conduction System Cells through cAMP Signaling

(A) A proposed mechanism of SN action. cAMP activators and inhibitors are shown in red and green, respectively.

(B) Quantification of the immunofluorescence staining of Cntn2:EGFP expression by MetaExpress image analysis. Cells were treated with cAMP activators (ZA, cAMP, and FSK) or inhibitor (H89), respectively. A blue column (DMSO control), pink column (SN treatment), orange columns (cAMP activators or inhibitors treatment), and purple columns (treatment of cAMP activators or inhibitors combined with SN) are shown.

(C) Quantification of cAMP levels. Error bars show SD. Comparison of either the activator or the inhibitor treatments with DMSO control is indicated by an asterisk (^∗). Comparison of either activators or inhibitors combined with SN to the activators or inhibitors itself is shown by a hashtag (#). Results are derived from three independent experiments.

Statistical significance is indicated: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 and ^#p < 0.05, ^##p < 0.01, ^###p < 0.001.