Skip to main content
. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1256–1272. doi: 10.1021/bi061466i

FIGURE 1.

FIGURE 1

Expression of MREG and immunoprecipitation from OS extracts. (A) Western blot of human ARPE-19 cells cultured and isolated in the light, ARPE-19 (L), or in the dark, ARPE-19 (D), and rat RPE-J cells and bovine retinal homogenate immunoblotted with anti-MREG mAb165. (B) Cell lysates from the indicated cell lines were also analyzed by Western blotting using anti-MREG mAb165. (C) Western blot of purified ROS extracts, His–MREG, and E. coli-expressed GST fusion proteins using anti-MREG mAb126 and anti-MREG mAb165. (D) Peripherin-2 interacts with MREG. Anti-MREG mAb165 was used to immunoprecipitate protein from ROS extracts, and the IP products were detected with anti-rom-1 mAb1C6 or anti-peripherin-2 mAb2B6 as described in Methods.