FIGURE 10.

Immunogold labeling of MREG and rhodopsin in mouse photoreceptors. Ultrathin sections of LR White-embedded wild-type C57BL6 mouse retina, immunogold labeled with anti-MREG mAb126 antibody (6 nm gold particles) and anti-opsin antibody (12 nm gold particles). (A) Section labeled with the MREG antibody. Clusters of label are evident throughout the inner segments (bottom portion). The outer segments (top portion) are not labeled. The scale bar is 1 μm. (B) Higher magnification of a section double labeled with both MREG and opsin antibodies. The area shown is at the base of the cilium; the basal bodies are evident. The arrows point to single gold particles indicating opsin labeling. The opsin labeling and melanoregulin labeling do not coincide. The scale bar is 300 nm.