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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1256–1272. doi: 10.1021/bi061466i

FIGURE 2.

FIGURE 2

(A) Immunolocalization of MREG in mouse retinas. Sections (7 μm) of fixed 4-month-old mouse WT (CB6F1/J), rds+/−< < and rds–/– retinas were stained with either anti-peripherin-2 mAb5H2 to mark outer segments (A) or anti-sodium potassium ATPase to detect the apical region of RPE (B) followed by anti-mouse Alexa Fluor 647 secondary antibody (colored red) and double labeled with anti-melanoregulin mAb126 conjugated to Alexa Fluor 594 (colored green). Nuclei were visualized with DAPI. Images were captured on a Bio-Rad Radiance 2100 laser scanning confocal microscope (Bio-Rad) using the same laser settings: RPE, retinal pigment epithelium; OS, outer segment; IS, inner segment; and ONL, outer nuclear layer. The scale bar is 5 μm. Arrows indicate colocalization of MREG and sodium potassium ATPase.

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