FIGURE 3.

MREG inhibition of R₁₈ plasma membrane–target membrane fusion. Fusion was assessed as an increase in R₁₈ fluorescence over time as R₁₈PM fuses with either disk membranes preincubated with the indicated amounts of MREG at 37 °C for 30 min (○) or peripherin-2-enriched rim specific vesicles preincubated with the indicated amounts of MREG (●). The amount of fusion was measured at 37 °C as described in Methods and is presented as the percent of the total fusion. Data represent two independent experiments with fusion assays performed in triplicate.