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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1256–1272. doi: 10.1021/bi061466i

FIGURE 5.

FIGURE 5

Binding of MREG to PerCter assessed by surface plasmon resonance. (A) Overlay of sensorgrams showing that immobilized peripherin-2 interacts with MREG, OS extracts, and a PerCter specific mAb, mAb2B6. PerCter does not interact with vesicles or OS PM extracts. (B) Overlay of sensorgrams showing the interaction of decreasing concentrations of MREG with immobilized PerCter. Data points were collected every 0.2 s. (C) Representative overlay of sensorgrams showing the interaction of decreasing concentrations of PerCter with immobilized MREG. The thin arrow indicates the response with just GST without peripherin-2. Data points were collected every 0.2 s.

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