FIGURE 9.

Analysis of protein distribution in the retina by Western blotting of serial tangential sections. (A) Thin rat retina cross section counterstained with toluidine blue (courtesy of A. Michaud, Massachusetts Eye and Ear Infirmary, Boston, MA). Retinal layers are as follows: ROS, rod outer segments; RIS, rod inner layer. (B) Rat retina was flat-mounted, frozen, and serially 5 μm sectioned. The protein content of each section was analyzed by Western blotting using antibodies against proteins indicated on the left of each blot. (C) Fluorescence of MREG (●) Per 2 (○) and rhodopsin (· · ·) bands in each section plotted as a percentage of a combined fluorescence in all sections. The data are taken from one of three similar experiments.