. Author manuscript; available in PMC: 2015 Jun 18.

Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1256–1272. doi: 10.1021/bi061466i

Table 1.

Primers Used in the Generation of the Bovine Peripherin-2 and ROM-1 Constructs^a

mutant	primer	sequence
PerCter scrambled helix	P1 (F)	TGGAGAAGAGCGTGCCGGAGGCCTGGGGGGCCACTCTGGAGAGTGTGAA
	P2 (R)	CTCCGGCACGCTCTTCTCCAGAAGCCAGCC
	P3 (F)	GTGTGAAGAAGCTGGGCAAGGACAACTACGTGCTAGCCGAGGGCGAG
	P4 (R)	CTTGCCCAGCTTCTTCACACTCTCCAGAGT
ROM-1Cter	P5 (F)	ATTAGGATCCCGGTACCTGCAGACAGCAC
	P6 (R)	ATTAGGATCCTCACTAGGCCTCAGGCAGACACTC
PerNter	P7 (F)	ACAAGGATCCATGGCGCTGCTGAAAGTCAAATTTGACCA
	P8 (R)	ATTAGGATCCTCAGCCTGCCGCCGGGGC

Forward (F) and reverse (R) primers are given.