Table 2.
Primers Used in the Construction of the Peripherin-2 C-Terminal Deletion Mutantsa
| mutant | primer | sequence | PCR product size (bp) |
|---|---|---|---|
| M1 | P1 (F) | AATATCTAGATCATGCCGCCGGGGCCT | 189 |
| P2 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M2 | P3 (F) | GACGTCTAGATCACGCCGGGGCCT | 186 |
| P4 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M3 | P5 (F) | GAGCTCTAGATCACGGGGCCTGGCCT | 183 |
| P6 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M4 | P7 (F) | GATCTCTAGATCAGGCCTGGCCTGCGT | 180 |
| P8 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M5 | P9 (F) | GAGGTCTAGATCACTGGCCTGCGTCCTC | 177 |
| P10 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M6 | P11 (F) | GAGGTCTAGATCAGCCTGCGTCCTCGC | 174 |
| P12 (R) | GAGCGGATCCCGCTACCTGCACAC | ||
| M7 | P13 (F) | GAGGTCTAGATCATGCGTCCTCGCCCT | 171 |
| P14 (R) | GAGCGGATCCCGCTACCTGCACAC |
a
Forward (F) and reverse (R) primers are given.