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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Methods Mol Biol. 2006;332:169–179. doi: 10.1385/1-59745-048-0:167

Fig. 1.

Fig. 1

Cdt toxin treatment alters the buoyant density of Jurkat cell membrane rafts. In order to determine whether the toxin, or any of its subunits, localize to lipid microdomains, detergent-resistant membranes (DRMs) from both untreated Jurkat cells and cells exposed to CdtABC were isolated. After a 2-h incubation, Jurkat cells were disrupted, homogenized in ice-cold Triton X-100, and separated on a sucrose gradient. Two distinct low-buoyant density bands, designated DRM1 and DRM2, were obtained and the position the sucrose density gradient determined as a measure of refractive index as indicated. The cholesterol content composition of these bands was analyzed (24) and is presented as percentage of the total membrane cholesterol.