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. 2015 Jun 1;26(11):2030–2043. doi: 10.1091/mbc.E14-12-1597

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© 2015 Singh et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Internalized NHE3 colocalizes with SNX27 in EEA-1– and Rab5-containing vesicles near the plasma membrane. (A) Biochemical internalization assay of HA-NHE3 in SK-CO15 cells. Representative examples of fluorescence localization pattern of endogenous SNX27 (red) and internalized NHE3 (green). The inset shows a magnified image of the boxed area. (B, C). Cellular localization of endogenous SNX27 (green) and EEA1 (red) in SK-CO15 and HEK293 cells, respectively. (D) HEK293 cells were transfected with early endosome marker GFP-rab5a or (E) recycling marker GFP-rab4a or (F) GFP-rab11a. The colocalization of endogenous SNX27 (green) and endosomal marker (red) was determined with anti-SNX27 antibody and anti-GFP antibodies. Scale bar, 10 μm. Representative images from six or seven independent experiments with similar results.