FIGURE 6:

SNX27 is necessary for serum to rapidly stimulate NHE3 activity. (A) SKCO-15 cells expressing control shRNA or SNX27 shRNA were treated with 10% dialyzed fetal bovine serum for t = 0, 15, 30, or 60 min, followed by determination of Na⁺/H⁺ exchange activity as described. Data shown are means ± SE of three separate experiments. p < 0.05 values are compared with untreated control. (B) Postconfluent, serum-starved control shRNA and SNX27 shRNA SKCO-15 cells were incubated with NHS-acetate. Cells were then incubated with 10% dialyzed fetal bovine serum for 0, 15, 30, and 60 min at 37°C to allow exocytosis of NHE3 in response to serum. Cells were then chilled to 4°C, and newly inserted NHE3 (exocytosis of NHE3) was biotinylated and detected by Western blotting as described. A representative blot from three independent experiments with similar results.