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. 2015 Jun 1;26(11):2054–2066. doi: 10.1091/mbc.E14-10-1473

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© 2015 Guan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Intracellular single-color FCS calibration. (A) mTFP1 monomer and mTFP1-mTFP1 tandem dimer (GGGSGGGS linker) constructs. (B) Fluorescence image of mTFP1 monomer in single cells and fluorescence image of mTFP1-mTFP1 tandem dimer in single cells. (C) Representative intracellular FCS curves from cells with similar molecular number of mTFP1 and mTFP1-mTFP1. Insets show similar molecular number, but the intensity of mTFP1-mTFP1 cells is about double that of mTFP1. (D) Representative intracellular FCS curves from cells with similar fluorescence intensities of mTFP1 and mTFP1-mTFP1. Insets show similar fluorescence intensity, but the molecular number of mTFP1-mTFP1 cells is about half that of mTFP1. (E) Comparison of average intracellular molecular brightness of mTFP1 and mTFP1-mTFP1.