Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 1;26(11):2054–2066. doi: 10.1091/mbc.E14-10-1473

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Guan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 6: — Intracellular induced dimerization measured by FCCS. (A) Schematic of induced heterodimerization of hmKeima8.5-FKBP12 and mTFP1-FRB by rapamycin. (B) Cross-correlation curves, comparison before and after addition of rapamycin. (C) Rapamycin induced dimerization measured by the percentage of dual-color molecules over total molecule number of hmKeima8.5. The binding of FKBP12 and FRB causes increase in dual-color percentage. A control construct (mTFP1-P2A-hmKeima8.5) under rapamycin addition is unchanged. (D) Time course of heterodimerization in individual cells upon addition of different concentrations of rapamycin. (E) Two cross-correlation curves from cell 1 at 45 and 75 min after treatment with 100 nM rapamycin. (F) Schematic of association of Coumarin 343–conjugated monofunctional binder (SAS121) with FKBP12-hmKeima8.5, followed by competition with excess nonfluorescent monofunctional binder (MAZ1258). (G) FCCS Measurements show the complex of SAS121 with FKBP12-hmKeima8.5 and dissociation upon excess addition of MAZ1258.