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. 2015 Jun 1;26(11):2080–2095. doi: 10.1091/mbc.E15-02-0073

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© 2015 Zorbas, Nicolas et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — The 18S rRNA m⁷G and modifications are conserved in S. cerevisiae and Homo sapiens. (A) Secondary structure of the 18S rRNA. The insets illustrate conservation of rRNA sequence and secondary structure near the N⁷-methylguanosine (m⁷G) modification introduced by Bud23-Trm112 in yeast and WBSCR22-TRMT112 in humans and in the vicinity of the two N⁶,N⁶-dimethyladenosines synthesized by Dim1 in yeast and DIMT1L in humans. Modified nucleotide positions are highlighted in red. The 5′, central (C), 3′ major (3′ M), and 3′ minor (3′ m) domains are indicated. (B) Three-dimensional representation of the yeast small subunit (model based on Protein Data Bank entry 3U5B) with posttranscriptional modifications and functional sites highlighted. The decoding site (DCS, in cyan) at the base of helix 44 (in anthracite) and the mRNA entry (green circle) and exit (red circle) sites are indicated. Residues shown as green and red spheres are 2′-O methylated or pseudouridylated, respectively. Bd, body; Bk, beak; H, head; Lf, left foot; Nk, neck; Pt; platform, Rf, right foot; Sh, shoulder.

Inline graphic — The 18S rRNA m⁷G and modifications are conserved in S. cerevisiae and Homo sapiens. (A) Secondary structure of the 18S rRNA. The insets illustrate conservation of rRNA sequence and secondary structure near the N⁷-methylguanosine (m⁷G) modification introduced by Bud23-Trm112 in yeast and WBSCR22-TRMT112 in humans and in the vicinity of the two N⁶,N⁶-dimethyladenosines synthesized by Dim1 in yeast and DIMT1L in humans. Modified nucleotide positions are highlighted in red. The 5′, central (C), 3′ major (3′ M), and 3′ minor (3′ m) domains are indicated. (B) Three-dimensional representation of the yeast small subunit (model based on Protein Data Bank entry 3U5B) with posttranscriptional modifications and functional sites highlighted. The decoding site (DCS, in cyan) at the base of helix 44 (in anthracite) and the mRNA entry (green circle) and exit (red circle) sites are indicated. Residues shown as green and red spheres are 2′-O methylated or pseudouridylated, respectively. Bd, body; Bk, beak; H, head; Lf, left foot; Nk, neck; Pt; platform, Rf, right foot; Sh, shoulder.