Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 1;26(11):2128–2138. doi: 10.1091/mbc.E14-11-1552

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Herrador et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 2: — Phosphorylation of Rim8. (A) Effect of ambient pH on Rim8 electrophoretic mobility. Protein extracts from strain OVY247 (RIM8-3HA) prepared before and after a shift from pH 3.5 to 7 were prepared by glass bead disruption and immunoblotted with anti-HA (top) or anti-actin (bottom) antibodies. (B) Phosphatase assay. Y04414 (rim8∆) was transformed with pHA-Rim8 (top) or a C-terminal truncated derivative (residues 1–460; bottom), grown to mid log phase, and shifted to pH 7.5. Anti-HA–immunoprecipitated protein extracts were treated with λ phosphatase (λPPase) with or without phosphatase inhibitor and immunoblotted with anti-HA antibody. Positions of the different Rim8 forms (P, phosphorylated; Ub, ubiquitinated) are indicated. (C) Rim21-dependent phosphorylation of Rim8. OVY247 (RIM8-3HA), OVY270 (rim21∆ RIM8-3HA), and OVY271 (rim101∆ RIM8-3HA) were grown to mid log phase (final pH 3.5), and protein extracts were immunoblotted with anti-HA (top) or anti-actin (bottom) antibodies.