TABLE 2:
Plasmids used in this study.
| Plasmid | Expressed protein; vector | Experiment | Reference/source |
|---|---|---|---|
| pNXgate-Rim8 | NubG-3HA-Rim8; pNXgate | mbSUS | This study |
| pNXgate-Rod1 | NubG-3HA-Rod1; pNXgate | mbSUS | This study |
| MetYCgate-Rim21 | Cub-PLV-Rim21; pMetYCgate | mbSUS | This study |
| MetYCgate-Dfg16 | Cub-PLV-Dfg16; pMetYCgate | mbSUS | This study |
| MetYCgate-Rim9 | Cub-PLV-Rim9; pMetYCgate | mbSUS | This study |
| pLexA-HA-Rim8 | LexA-HA-Rim8; pLexA(1-202)+PL | Two-hybrid | Herrador et al. (2010) |
| pLexA-HA-Rim8-S76P | LexA-HA-Rim8-S76P; pLexA(1-202)+PL | Two-hybrid | This study |
| pGAD-Rim21CT | GAD-Rim21(327-533); pACT2 | Two-hybrid | Herrador et al. (2010) |
| pGAD-Dfg16CT | GAD-Dfg16(426-619); pACT2 | Two-hybrid | This study |
| pGAD-Vps23 | GAD-Vps23; pACT2 | Two-hybrid | Herrador et al. (2010) |
| pHA-Rim8 | HA-Rim8; pRS313 | Western blot | Herrador et al. (2010) |
| pHA-Rim8-K521R | HA-Rim8-K521R; pRS313 | Western blot | Herrador et al. (2010) |
| pHA-Rim8-S76P | HA-Rim8-S76P; pRS313 | Western blot | This study |
| pHA-Rim8(1-460) | HA-Rim8(1-460); pRS313 | Western blot | This study |
| pHA-Rim8(1-460)-S76P | HA-Rim8(1-460)-S76P; pRS313 | Western blot | This study |
| pHA-Rim8-1S/A | HA-Rim8-S232A; pRS313 | Western blot | This study |
| pHA-Rim8-3S/A | HA-Rim8-SSS(231-233)AAA; pRS313 | Western blot | This study |
| pHA-Rim8-17ST/A | HA-Rim8-17ST(231-256)A; pRS313 | Western blot | This study |
| pHA-Rim8-17ST/D | HA-Rim8-17ST(231-256)D; pRS313 | Western blot | This study |
| pFlag-Rim8 | Flag-Rim8; pRS313 | Western blot | Herrador et al. (2010) |
| pFlag-Rim8-17ST/A | Flag-Rim8-17ST(231-256)A; pRS313 | Western blot | This study |
| pFlag-Rim8-17ST/D | Flag-Rim8-17ST(231-256)D; pRS313 | Western blot | This study |
| pADH1-HA-Rim8 | HA-Rim8; pRS313 | Western blot | This study |
| pADH1-Flag-Rim21CT | Flag-Rim21(327-533); pWS93 derivative | Western blot | This study |
| pADH1-Flag-Rim21CT(L) | Flag-Rim21(327-533); pSK134 derivative | β-Galactosidase assays | This study |
| pADH1-RFP-Rim21CT | RFP-Rim21(327-533); pWS93 derivative | Fluorescence microscopy | This study |
| pADH1-Flag-Dfg16CT | Flag-Dfg16(426-619); pWS93 derivative | Western blot | This study |
| pGST-Rim8(1-393) | GST-Rim8(1-393); pGEX-5X-1 | In vitro phosphorylation | This study |
| pGST-Rim8(1-393)-17ST/A | GST-Rim8(1-393)-17ST(231-256)A; pGEX-5X-1 | In vitro phosphorylation | This study |
| pCUP1-His7-Yck1 | His7-Yck1; pYEX4T-1 derivative | In vitro phosphorylation | This study |
| pCUP1-His7-Yck1-K98R | His7-Yck1-K98R; pYEX4T-1 derivative | In vitro phosphorylation | This study |
| pRim21-HA | Rim21-HA; pRS315 | Western blot | This study |
Vectors: pNubWT-2, pNXgate, and pMetYCgate (Obrdlik et al., 2004); pLexA(1-202)+PL (Ruden et al., 1991); pACT2 (Clontech); pRS313 and pRS315 (Sikorski and Hieter, 1989); pGEX-5X-1 (GE Healthcare); pYEX4T-1 (Clontech), pWS93 (Song and Carlson, 1998); pSK134 (Vincent and Carlson, 1999).