TABLE 1:
Summary of Gsk-3–dependent DMR validation by bisulfite sequencing.
| Locus | DMR genomic coordinates | WT% CpG | DKO% CpG | Fraction |
|---|---|---|---|---|
| Pax3 | chr1:78191696-78192257 | 51.8 | 15 | 2 M |
| En1 | chr1:122496266-122497436 | 45.3 | 18.7 | 2 M |
| Blcap/Nnat | chr2:157385054-157386455 | 77.4 | 8.1 | 2 M |
| Gnas | chr2:174120416-174122205 | 63.6 | 5.8 | 2 M |
| Gnas | chr2:174122344-174123811 | 43.2 | 18.7 | 2 M |
| Gnas | chr2:174123824-174124999 | 36.8 | 8.7 | 2 M |
| Gnas | chr2:174125041-174126654 | 67.3 | 17.5 | 2 M |
| Adar | chr3:89471020-89471670 | 80.4 | 15.6 | 2 M |
| Klf4 | chr4:55540960-55541232 | 86.3 | 13.0 | 2 M |
| Cdc42 | chr4:136927927-136928474 | 69.0 | 35.6 | 2 M |
| Fkbp6 | chr5:135725089-135726550 | 88.8 | 48.7 | 600 mM |
| Mest/Copg2 | chr6:30682552-30683326 | 80.7 | 13.0 | 2 M |
| Mest/Copg2 | chr6:30685155-30685990 | 86.3 | 19.1 | 2 M |
| Peg3 | chr7:6680997-6682243 | 44.4 | 1.1 | 600 mM |
| H19 | chr7:149825745-149826024 | 47.2 | 24.4 | 600 mM |
| Kcnq1 | chr7:150480888-150482586 | 90.7 | 35.1 | 2 M |
| Kcnq1 | chr7:150491439-150492052 | 97.1 | 87.2 | 600 mM |
| Kcnq1 | chr7:150526408-150527088 | 89.0 | 75.3 | 600 mM |
| Mycn | chr12:12943555-12944660 | 54.2 | 13.0 | 2 M |
| Begain | chr12:110311312-110311914 | 81.2 | 24.3 | 2 M |
Genomic loci and chromosomal coordinates of each DMR. Also shown is the percentage of DNA methylation at each validated DMR in WT and Gsk-3 DKO ESCs and the fraction in which the DMR was identified (2 M or 600 mM). All p < 0.05.