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. 2015 Jun 12;9:3003–3016. doi: 10.2147/DDDT.S81656

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© 2015 Cui et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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OPN deficiency impaired the induction of polarized Th1 responses to HBV antigens through DCs.

Notes: T-cells were isolated from the WT and OPN^−/− mice spleen by MACS and cocultured with CD11c⁺ cells isolated from the cell derived from murine BM of WT mice and OPN^−/− mice after HBV antigenic stimulations at a ratio of 10:1, respectively, and then cytokines levels in the coculture supernatants were detected by ELISA. The levels of IFN-γ (A), TNF-α (B), IL-4 (C) and IL-10 (D) in the coculture supernatants are compared. *P<0.05; **P<0.01. ***P<0.001.

Abbreviations: OPN, osteopontin; DCs, dendritic cells; HBV, hepatitis B virus; BM, bone marrow; WT, wild-type; MACS, magnetically activated cell sorting; HBcAg, hepatitis B core antigen; HBsAg, surface antigen of the hepatitis B virus; IL, interleukin; TNF, tumor necrosis factor; IFN, interferon; BMDC, bone marrow-derived dendritic cell; ELISA, enzyme-linked immunosorbent assay.