Figure 3.

Membrane topology probed for the ¹⁵N Notch1 TM/JM segment in 15% DMPC/DH₆PC bicelles (q = 0.33) at pH 5.5 and 318 K. A plot of the ratio of site-specific peak intensities from samples containing a paramagnet vs those from a diamagnetic reference sample reveals the probe accessibility of each residue. The Gd(III)-DTPA accessibility is colored blue and the 16-DSA accessibility red. The bottom panel represents a similar plot using 3-cyano-PROXYL. Sites with negative bars represent either proline residues or instances in which extensive peak overlap prevented reliable analysis.