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Translation of RRM1 from wild-type (wt) and modified RRM1 mRNA (TAG codon at the position corresponding to Ala35) by using an S-30 system prepared from ribosomal clone 040329 in the presence of 3(S)-methyl-β-alanyl-tRNACUA (β-mAla; 4) (lanes 1 and 2) or in the absence of any tRNACUA (lane 3).
