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. 2015 May 18;54(23):3694–3706. doi: 10.1021/acs.biochem.5b00389

Table 2. IC50 Assay for Verifying the Sensitivity of the Modified Ribosomes to β-Puromycin Derivatives in the Presence of Erythromycin (2.5 μg/mL).

  sequence in 23S rRNA of the modified ribosome
 IC50 (μg/mL)a
clone region 1 region 2 β3-puromycin β2-puromycin
010335 2057UGCGUGG2063 2502AGAUGA2507 163 ± 17 71 ± 10
010374 2057UGCGUGG2063 2502CGCUCG2507 125 ± 7 59 ± 5
a

Each number represents the average of three independent experiments ± the standard deviation.