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| Gene | DNA sequence (5′–3′) | |
| Sox-9 | Forward | GCCAGGTGCTCAAAGGCTA |
| Reverse | TCTCGTTCAGAAGTCTCCAGAG | |
| Col2α1 | Forward | ACACTGGGACTGTCCTCTG |
| Reverse | GTCCAGGGGCACCTTTTTCA | |
| Col10α1 | Forward | ATGCTGCCACAAATACCCTTT |
| Reverse | GGAATGAAGAACTGTGTCTTGGT | |
| IGF-1 | Forward | TGGATGCTCTTCAGTTCGTG |
| Reverse | CACTCATCCACAATGCCTGT | |
| IGF-2 | Forward | CTTCTCCTCCGATCCTCCTG |
| Reverse | TGAGAAGCACCAACATCGAC | |
| β-actin | Forward | GTTGTCGACGACGAGCG |
| Reverse | GCACAGAGCCTCGCCTT |
