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Osteoblast population increases significantly from 7 days post injury. (a) IHC analysis was performed with anti-GFP antibody on the injury-adjacent tissues. Arrowheads indicate osteoblasts at the periosteum region. The section at day 14 was co-stained with alcian blue, and the arrow indicates cartilage; (b) IHC was performed with anti-GFP antibody on the injury sites. Arrows indicate the GFP+ mature osteoblasts. The boxed regions are shown in higher magnifications.
