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. 2015 May 15;34(1):49. doi: 10.1186/s13046-015-0170-5

Fig. 4.

Fig. 4

Effect of SNORD78 overexpression on NSCLC cell viability and cell cycle. (a) qRT-PCR analysis of SNORD78 expression following transfection of A549 cells with SNORD78. (b) A549 cells were transfected with SNORD78 or control. CCK8 assays were performed to determine the proliferation of A549 cells. (c) Cell cycle analysis determined the relative cell numbers in each cell-cycle phase after propidium iodide staining of SNORD78 overexpressed A549 cells. Numbers inside bars represent percentages of cells in each phase. (d) qRT-PCR analysis of GAS5 expression following transfection of A549 cells with SNORD78. Data represent the mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01