Fig. 5.

SNORD78 promoted invasion of NSCLC cells via inducing epithelial-mesenchymal-transition (EMT). (a) H1975 cells were transfected with shRNA control or shRNA SNORD78. Transwell assays were performed to investigate the invasive ability of H1975 cells. Data represent the mean ± S.D. from three independent experiments. (b) A549 cells were transfected with LV-control or LV-SNORD78. Transwell assays were performed to investigate the invasive ability of A549 cells. Data represent the mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01. (c) immunofluorescence images of H1975 cells stained using antibodies against E-cadherin, Vimentin or N-cadherin after transfected with shRNA control or SNORD78 shRNA-2 (original magnification × 200). (d) Western-blot analysis of phenotypic markers after SNORD78 knockdown in H1975 cells. Relative protein expression was identified (n = 3) and normalized to β-actin. Data represent the mean ± S.D.. (e) Results of MSP analysis of E-cadherin gene in SNORD78 shRNA-2-transfected H1975 cells, and control cells. M and U, PCR products of methylated and unmethylated alleles, respectively