Fig. 6.

SNORD78 is required for the self-renewal of cancer-stem cells of NSCLC. (a) CD133 positive cells were isolated from NSCLC cells A549. (b) There was a high level of expression of SNORD78 in CD133+ cells versus CD133- cells of A549 cells as determined by qPCR analysis. (c) Representative images of mammospheres formed from A549 cells. Inhibition of SNORD78 reduced the number of mammospheres (original magnification × 100). (d) Smaller sizes of mammospheres were observed after SNORD78 knockdown in comparison with control (original magnification × 400). Representative images of mammospheres were shown above. Experiments were independently repeated at least three times. (e) Western-blot analysis of stemness factors after SNORD78 knockdown in H1975 cells. Relative protein expression was identified (n = 3) and normalized to β-actin. Data represent the mean ± S.D..*, p < 0.05; **, p < 0.01