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The degree of nuclear localization of hPPIP5K2-GFP is independent of its degree of expression. Either (panel a) live, or (panel b) fixed, HEK293T cells that had been transfected with hPPIP5K2-GFP were treated with the nuclear DRAQ5 stain. The scatter plots show the total GFP fluorescence in each individual cell plotted against the same cell’s Similarity Score. The values of the correlation coefficients (r) are shown in each panel
