Fig. 8.
Comparison of the degree of nuclear localization of hPPIP5K2NLS-3A-GFP and hPPIP5K2NLS-3A/S1006A-GFP. Shown is an analysis by imaging flow cytometry of HEK293T cells that were transfected with either a, hPPIP5K2NLS-3A-GFP (n = 555; 75893 ± 3073 total fluorescence units) or b hPPIP5K2NLS-3A/S1006A-GFP (n = 1328; 83844 ± 1716 total fluorescence units), and then stained with DRAQ5. The histograms depict the frequency of binned. Similarity Scores denoting the degree of co-localization of the GFP signal and nuclear stain, derived on a cell-by-cell basis. Best-fit Gaussian plots are also shown