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. 2015 Apr 9;70(7):1946–1959. doi: 10.1093/jac/dkv060

Figure 1.

Figure 1.

Accumulation of tetracycline in S. pneumoniae. (a) Accumulation of labelled tetracycline was measured in S. pneumoniae R6 WT, R6M1TC-5 and R6M2TC-4 at baseline and at 5 min intervals for a total of 20 min. (b) The role of mutations selected at the patA locus in R6M1TC-5 in accumulation of tetracycline was assessed by comparing the accumulation of labelled tetracycline in R6 WT, R6patA(R6M1TC), R6p_patA(R6M1TC)-rpsL+ and R6CDSpatA(R6M1TC)-rpsL+ for up to 20 min. (c) The role of mutations selected at the patA locus in R6M2TC-4 in accumulation of tetracycline was assessed by comparing the accumulation of labelled tetracycline in R6 WT, R6patA-patB(R6M2TC), R6p_patA(R6M2TC)-rpsL+ and R6CDSpatA-patB(R6M2TC)-dhfr+ for up to 20 min. Results represent the means of three independent replicates. A t-test was performed on normalized data at 10 and 20 min and the significance threshold set to P ≤ 0.05. (d) Accumulation of tetracycline in the presence of reserpine in R6 WT, R6patA(R6M1TC) and R6patA-patB(R6M2TC). Reserpine (20 mg/L) was added at 15 min.