Table 1.
Transport studies in Xenopus laevis oocytes
| SLC10A4 | Control | Ratio | |
|---|---|---|---|
| Uptake (pmol/oocyte/60 min) | |||
| [3H]Histamine (10 µM) | 0.29 ± 0.02 | 0.27 ± 0.01 | 1.0 |
| [3H]Aspartate (1 µM) | 0.22 ± 0.01 | 0.28 ± 0.01 | 0.8 |
| [3H]Acetate (25 µM) | 8.79 ± 0.51 | 8.36 ± 0.72 | 1.0 |
| [3H]Choline (1 µM) | 2.68 ± 0.52 | 2.20 ± 0.19 | 1.2 |
| [3H]GABA (1 µM) | 0.52 ± 0.05 | 0.47 ± 0.04 | 1.1 |
| [3H]Norepinephrine (1 µM) | 0.16 ± 0.01 | 0.21 ± 0.02 | 0.8 |
| [3H]Glutamate (1 µM) | 0.014 ± 0.001 | 0.014 ± 0.002 | 1.0 |
| Uptake (pmol/oocyte/30 min) | |||
| [3H]Acetylcholine (10 µM) | 43.51 ± 3.62 | 37.51 ± 3.67 | 1.2 |
| [3H]Dopamine (10 µM) | 1.36 ± 0.06 | 1.43 ± 0.05 | 1.0 |
| [3H]PREGS (1 µM) | 1.19 ± 0.09 | 1.06 ± 0.09 | 1.1 |
| [3H]DHEAS (1 µM) | 0.043 ± 0.002 | 0.043 ± 0.004 | 1.0 |
| [3H]E-3-S (1 µM) | 0.050 ± 0.001 | 0.060 ± 0.005 | 0.8 |
| Uptake (pmol/oocyte/10 min) | |||
| [3H]Lithocholic acid (0.3 µM) | 3.68 ± 0.23 | 4.13 ± 0.07 | 0.9 |
| [3H]Taurocholic acid (0.3 µM) | 0.0158 ± 0.0009 | 0.0159 ± 0.0007 | 1.0 |
| [3H]ATP (5 mM) | 70.66 ± 9.06 | 68.84 ± 8.26 | 1.0 |
| [35S]Adenosine 5`-(γ-thio) triphosphate (5 mM) | 69.76 ± 4.36 | 67.73 ± 2.53 | 1.0 |
Xenopus laevis oocytes were injected with human SLC10A4 cRNA or with water (control). Values represent uptake of the indicated compound as mean ± SD of at least 10 oocytes per group. Ratio represents uptake into SLC10A4 cRNA-injected oocytes divided by uptake into water-injected oocytes.