Table 2.
Transport studies in HEK293 cells
| Compound (5 µM) | NTCP | SLC10A4 | NtNTCP-SLC10A4-CtNTCP | |||
|---|---|---|---|---|---|---|
| Uptake | Ratio | Uptake | Ratio | Uptake | Ratio | |
| [3H]E-3-S | 276.93 ± 29.12 | 10.1* | 35.23 ± 2.01 | 1.3 | 27.91 ± 1.38 | 1.0 |
| [3H]DHEAS | 225.12 ± 16.51 | 6.9* | 38.22 ± 0.90 | 1.2 | 31.84 ± 1.38 | 1.0 |
| [3H]PREGS | 1,656.09 ± 201.01 | 4.7* | 366.06 ± 22.23 | 1.0 | 383.62 ± 17.38 | 1.0 |
| DAT | SLC10A4 | NtNTCP-SLC10A4-CtNTCP | ||||
|---|---|---|---|---|---|---|
| [3H]Dopamine | 1,578.17 ± 107.47 | 13.2* | 128.61 ± 16.60 | 1.1 | 157.92 ± 19.11 | 1.3 |
| [3H]Glutamate | 22.60 ± 2.95 | 1.4 | 13.57 ± 1.62 | 0.8 | ||
| [3H]Histamine | 26.24 ± 1.40 | 1.3 | 25.68 ± 0.63 | 1.3 | ||
| [3H]Acetyl-choline | 352.00 ± 38.76 | 1.0 | 307.64 ± 31.41 | 0.9 | ||
| [3H]Choline | 929.53 ± 30.50 | 1.1 | 866.83 ± 23.63 | 1.0 | ||
Values represent uptake in pmol/mg protein/30 min of the indicated compound as mean ± SD of quadruplicate determinations. Ratio represents uptake into carrier-expressing HEK293 cells divided by uptake into mock-transfected HEK293 cells. * Significantly different from control with p < 0.01.