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. 2015 Jun 19;15:474. doi: 10.1186/s12885-015-1445-0

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© SP et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Combination MSM-Tam treatment synergistically inhibited the Jak2/STAT5b signaling pathway. (a) Western blotting analysis of cytoplasmic protein levels in MCF-7 and T47D cells, and after treatment with Tam, MSM, or their combination for 24 h. (b) Nuclear protein level analysis in MCF-7 and T47D cells, and after treatment with Tam, MSM, or the drug combination for 24 h using western blotting. (c) The DNA binding activity of STAT5b was inhibited by the drug combination, as analyzed by a gel shift assay in MCF-7 cells. (d) The DNA binding activity of STAT5b was inhibited by the drug combination and confirmed by a ChIP assay in MCF-7 cells. Statistical analyses were conducted using the ANOVA test (**P < 0.01 and ***P < 0.001)