Table 1.

RT-PCR primers sequences used for the amplification of multiple human cDNAs

Sl No	Gene	Annealing temperature (°C)	Product size (bp)	Sequence (5’ - 3’)
1	Cyclin-D1	58	135	F – gctgcgaagtggaaaccatc
				R – cctccttctgcacacatttgaa
2	IGF-1Rβ	58	522	F – actatgccggtgtctgtgtg
				R – tgcaagttctgattgttgag
3	IGF-1	58	498	F – tcctcgcatctcttctacct
				R – tctggactcgccagtccaat
4	VEGF	58	405	F – aggagggcagaatcatcacg
				R – caaggcccacagggattttc
5	18S	58	490	F – agccttcggctgactggctgg
				R – ctgcccatcatcatgacctgg
6	MMP2	53	665	F – gagttggcagtgcaatacct
				R – gccatccttctcaaagttgt
7	MMP3	60	432	F – cctgctttgtcctttgatgc
				R – tgagtcaatccctggaaagt
8	MMP9	58	455	F – cctgccagtttccattcatc
				R – gccattcacgtcgtccttat
9	hIGF-1 (CHIP assay)	60	700	F – tggcatgttttgaggttttg
				R – gattggttgtgtggcatgag