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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Neurochem. 2015 Apr 8;134(1):86–96. doi: 10.1111/jnc.13096

Figure 1. Succinylation and acetylation of proteins in the cytosol and mitochondria are sensitive to inhibition of KGDHC.

Figure 1

Figure 1A. Characterization of the mitochondrial and cytosolic fractions. The media of cultured neurons was changed to BSS and the cells were incubated with or without 100 μM-CESP in BSS buffer for one hour at 37°C. Mitochondrial and cytosolic fractions were prepared. Ten μg protein were loaded on each lane and were separated on 4–20 % Tris-glycine gel and transferred to blotting membrane.

Figure 1B. Succinylation is sensitive to inhibition of KGDHC. Aliquots (10 μg protein) were added to each lane. Similar results were observed in three independent experiments.

Figure 1C. Acetylation is sensitive to inhibition of KGDHC. Aliquots (10 μg protein) were added to each lane.

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