FIGURE 3. SC inhibits isocitrate dehydrogenase (ICDH) activity.
Figure 3A. The inhibitory effects of SC varied with the amount of ICDH. The treatments were at 30°C for 50 min. The final ICDH activities were 4, 8, 16 or 32 mU/well. Values are means of two independent experiments.
Figure 3B. SC concentration dependent changes in ICDH. The amount of enzyme was kept at 8 mU and the amount of succinyl Co A was varied. ICDH was either not incubated or incubated for 40 min with the indicated concentration of SC and incubated at 30°C for 0 or 40 min. After incubation, the ICDH activity was assayed in a 96 well plate.