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Succinylation alters the activity of KGDHC. KGDHC was incubated with SC (100 μM) for either 0 min or 30 min in KGDHC lysate buffer. KGDHC (7.8 mU) was diluted in KGDHC lysate buffer and were read at 340–460 nm (cut off 455 nm) at 30°C for 30 min.
