TABLE 1. KG-dependent succinylation of KGDHC.
The 70 kDa bands in Figure 2A were cut and processed for mass spectrometry. This protein band contains many proteins. The main protein was albumin. The sequence of the residues that were succinylated are in Table 1. Each value was divided by an internal standard (the bottom line of the table) and then the plus KG was divided by the minus KG. We then divided the KG+ by KG-. When this is done for the standard the ratio is one.
Peptide sequence
|
Increased succinylation
|
---|---|
TPVSEKVTK | 5.4 |
KQTALVELLK | 3.1 |
LCVLHEKTPVSEK | 7.1 |
LCAIPSLR | 1.0 |