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. Author manuscript; available in PMC: 2016 Jun 16.
Published in final edited form as: Cell Rep. 2015 Jun 4;11(10):1638–1650. doi: 10.1016/j.celrep.2015.05.014

Figure 5. Puf3p(24A) phosphomutant forms punctate foci following glucose depletion.

Figure 5

Live cell imaging of cells expressing Puf3p-GFP or Puf3p(24A)-GFP using the CellASIC microfluidics platform, before and after switch to glucose depletion medium for the indicated times. Puf3p-GFP was uniformly distributed in the cytosol and not specifically localized to mitochondria regardless of glucose availability. Strikingly, Puf3p(24A) forms foci only after switch to glucose depletion medium. Approximately 40% of cells expressing Puf3p(24A)-GFP exhibited foci following glucose depletion, in contrast to 0% of cells expressing WT Puf3p-GFP. See also Figure S5 and Supplementary Videos. These foci exhibited partial co-localization with the p-bodies marker Dcp2p (Figure S5). Both Puf3p(24A) and Dcp2p formed foci only after switch to glucose depletion medium (Figure S5).