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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Chem Biol. 2015 May 25;11(7):504–510. doi: 10.1038/nchembio.1814

Figure 3. 15NO2− mediates glutathione nitrosation in the presence of •NO.

Figure 3

a-b, Representative LCMS/MS traces for GS14NO (a) and GS15NO (b) formation. c-f, Traces showing GS14NO and GS15NO formation from 2.5 μM MNO and 20 μM GSH in the absence (c) or presence of 20 μM (d), 200 μM (e) and 2 mM (f) 15NO2−. Traces are representative and reflect time-staggered replicate reactions (n=4). g, Total GSNO yields versus 15NO2− concentration. Data are mean ± SD (n=4), no statistical differences were found as determined by one way ANOVA. No GSNO was formed from 2 mM 15NO2− in the absence of MNO.