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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Chem Biol. 2015 May 25;11(7):504–510. doi: 10.1038/nchembio.1814

Figure 4. NO2− incorporation into NO2-CLA and GSNO is associated with symN2O3 formation.

Figure 4

a, Scheme illustrating the asymmetrical (1) and symmetrical (2) conformations of N2O3. Arrows indicate alternative bond cleavage patterns. Whereas asymN2O3 homolysis produces a unique set of products (1a, 1b), alternative cleavage of the O-N-O bonds in symN2O3 can be evidenced by isotopic labeling (blue and red represent 14N and 16O respectively, dark blue and green are 15N and 18O). b, Distribution of NO2-CLA isotopologues versus 15N18O2− concentration in the presence of 25 μM MNO and 20 μM CLA. c, Isotopic GSNO distribution versus 15N18O2− in the presence of 2.5 μM MNO and 20 μM GSH. Data for panels b and c are mean ± SD (n=4). Error bars are not distinguishable as they overlap with data points.